PyReconstruct Download

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PyReconstruct is a modern update of Reconstruct, a program developed in the lab of Prof. Kristen Harris by Dr. John Fiala (more info below). PyReconstruct was conceptualized and written in 2022-2024 by Harris Lab members Julian Falco and Michael Chirillo, MD, PhD. Like Reconstruct, PyReconstruct allows users to trace and annotate images, thereby creating 3D reconstructions. Among many other quality of life improvements, PyReconstruct can be used on Windows, Mac, and Linux machines. The user interface as well as data structures have been modernized and simplified. Questions can be directed to Dr. Chirillo (

System Requirements:

  • 256 MB RAM, 500 MHz CPU
  • Windows: Windows 10 and above
  • Mac: macOS 12 and above
  • Linux: X or Wayland windowing system
Further reading:

As with legacy Reconstruct, permission to use, copy, and redistribute PyReconstruct is granted freely under the terms of the GNU General Public License (version 3). We provide no warranty and the program's performance is the sole responsibility of the user. Please see more details in the GNU General Public License.


Reconstruct Download

Reconstruct (32-bit)
     with Help Manual and example.ser [Version, 2007]   

System Requirements:
  • Microsoft Windows (XP/Vista/7/8/10)
  • Pentium processor
  • 10 Mb of disk space for program files
  • at least 128 Mb of RAM
  • To use Reconstruct on a Mac, read these instructions.
Further reading:

Reconstruct was written by John C. Fiala, Ph.D.
For more info go to Reconstruct History and Citations.
Source code for Reconstruct is available on Github
For help with specific questions, contact the Reconstruct Yahoo User's Group or email the SynapseWeb admin at

Reconstruct was created with funding, in part, from the National Institutes of Health and the Human Brain Project under grants (P30 HD18655, R01 MH/DA 57351, and R01 EB 002170).

Permission to use, copy, and redistribute Reconstruct is granted without fee under the terms of the GNU General Public License version 2 as published by the Free Software Foundation.

(This software is distributed in the hope that it will be useful, but without any warranty, including the implied warranty of merchantibility or fitness for any particular purpose. For details read the terms of the GNU General Public License when installing the software.)