Introduction

Fig. 0.1. From Light Microscopy to Ultrastructure and Three-Dimensional Reconstructions: Pyramidal cells of neocortex are shown in first line, Purkinje cells of cerebellar cortex in second line, ganglion cells of trigeminal ganglion in third line. Standard hematoxyline-eosin staining (HE) is compared with cresyl violet staining (Nissl), silver impregnations (Golgi, Bielschowski) and toluidine blue stained epoxy resin semithin sections (Semi). Part of pyramidal cell perikaryon is shown on electron micrograph (EM) and appearance of dendrite of Purkinje cell is shown on three-dimensional reconstruction (3D).