Microwave Tissue Processing
Set up (can be done one day before)
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Epon:
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Label:
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Set out:
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Chemistry Preparation
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Solvents:
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Osmium:
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Uranyl Acetate:
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Microwave preparation:
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Processing
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Tissue transfer and rinse:
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Osmication:
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Rinse:
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Dehydration:
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END MICROWAVE PROCESSING AND SWITCH TO LONG-HAND |
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Infiltration:
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Embedding (the next day):
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Clean-up
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Waste containers:
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Glassware and equipment:
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Checklist:
power setting 1 (175W)
under vacuum
reduced osmium
1 min on/1min off/1 min on __ __
5 buffer rinses
osmium
1 min on/1min off/1 min on __ __
5 buffer rinses
2 water rinses
transfer to 50% ETOH
power setting 2 (250W)
no vacuum
dehydration
50% ETOH/UA 40s __
70% ETOH/UA 40s __
90% ETOH/UA 40s __
100% ETOH/UA 40s __
100% ETOH 40s __
END MICROWAVE PROCESSING
Infiltration (on rotator)
1:1 ETOH/PO 10 min_
1:2 ETOH/PO 10 min__
PO 15 min__ __
1:1 PO :epon 1 hour__
1:2 PO :epon O/N__
NEXT DAY
100% epon with DMP-30 1 hour
Tissue transferred via wooden applicator stick to coffin mold with apical dendrites at top, filled with epon with DMP-30, and placed in 60 C oven for 48 hours.